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pet-28(+) vector  (Millipore)


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    Structured Review

    Millipore pet-28(+) vector
    Pet 28(+) Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet-28(+)+vector/pet28a/pm38485022-69-6-8
    Average 90 stars, based on 1 article reviews
    pet-28(+) vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Modification:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Plasmid Preparation:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Polymerase Chain Reaction:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Mass Spectrometry:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Sequencing:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Purification:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Ligation:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Expressing:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Construct:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Generated:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    FLAG-tag:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).

    Amplification:

    Article Title: Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity
    Article Snippet: The M. musculus VDAC1 coding sequence was then inserted into an isopropyl β- d -1-thio galactopyranoside-inducible pET-28 vector (Novagen/EMD Millipore), with a C-terminal linker (Gly-Ser-Gly) followed by a 6×His purification tag, using ligation-independent cloning methods (Aslanidis & de Jong, 1990 ▸ ; primers are available upon request).

    Article Title: Analysis of the role of PCNA-DNA contacts during clamp loading
    Article Snippet: S. cerevisiae PCNA was cloned into a modified pET-28 vector (Novagen) so that it was preceded by an N-terminal 6-His tag cleavable by PreScissionTM protease.

    Article Title: A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Article Snippet: Please cite this article as: Danyelle Toyama, Mariana Abrahão Bueno de Morais, Felipe Cardoso Ramos, Letícia Maria Zanphorlin, Celisa Caldana Costa Tonoli, Augusto Furio Balula, Fernando Pellon de Miranda, Vitor Medeiros Almeida, Sandro Roberto Marana, Roberto Ruller, Mario Tyago Murakami, Flavio Henrique-Silva , A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).. The address for the corresponding author was captured as affiliation for all authors.. Please check if appropriate.

    Article Title: Transport of maternal transthyretin to the fetus in the viviparous teleost Neoditrema ransonnetii (Perciformes, Embiotocidae).
    Article Snippet: The molecular basis of viviparity in non-mammalian species has not been widely studied.. Neoditrema ransonnetii, a surfperch, is a matrotrophic teleost whose fetuses grow by ovarian cavity fluid (OCF) ingestion and by nutrient absorption via their enlarged hindgut.. We performed a proteomics analysis of N. ransonnetii plasma protein and found proteins specific to pregnant females; one of these was identified as transthyretin (TTR), a thyroid hormone distributor protein.

    Article Title: Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts
    Article Snippet: Genes corresponding to proteins of interest are commonly cloned into a pET-28 vector (Novagen) using standard PCR and molecular biology techniques.

    Article Title: Breakage of the oligomeric CaMKII hub by the regulatory segment of the kinase
    Article Snippet: For the mass spectrometry analyses, the human CaMKII-a hub domain (Uniprot_ID: Q9UQM7, resi- due 345–475) was cloned into a pET-28 vector (Novagen) that was modified to contain a PreScission Protease (Pharmacia) site between the N-terminal 6-histidine tag and the coding sequence, before being expressed in E. coli.

    Article Title: Proteins for the treatment of epithelial barrier function disorders
    Article Snippet: An expression construct was generated in which a pET-28 vector (Sigma Millipore) was used to harbor and express a polynucleotide sequence (SEQ ID NO:4) encoding SG-11 (SEQ ID NO:3) with a FLAG-tag (DYKDDDDK; SEQ ID NO:32) at the N-terminus of SG-11.

    Article Title: Structural basis for helicase-polymerase coupling in the SARS-CoV-2 replication-transcription complex
    Article Snippet: The coding sequences of the E. coli codon optimized SARS-CoV-2 nsp13 gene (gBlock from Integrated DNA Technologies) was cloned into a pET-28 vector (Novagen).



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